By Frank H. Stephenson

ISBN-10: 0128022116

ISBN-13: 9780128022115

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*Calculations in Molecular Biology and Biotechnology, 3rd Edition,* is helping researchers using molecular biology and biotechnology techniques―from pupil to professional―understand which kind of calculation to exploit and why. study in biotechnology and molecular biology calls for an enormous volume of calculations. result of one information set develop into the foundation of the subsequent. An errors of selecting the incorrect kind of equation can flip what may were a profitable examine venture or weeks of work and study right into a veritable condominium of playing cards. it may be the way you calculated the medium within which you attempt your pattern to calculating how lengthy it takes a pattern to develop to calculating the synthesis of a number of variables.

In one effortless to take advantage of reference, Stephenson experiences the math and records with regards to the day by day features of biotechnology and molecular biology labs, that's a sticking aspect for plenty of scholars, technicians, and researchers. The ebook covers the entire simple mathematical and statistical wishes for college students and execs, supplying them with a useful gizmo for his or her work.

- Features entire calculations in biotechnology and molecular biology experiments from begin to finish
- Provides insurance starting from simple medical notations to complicated matters like nucleic acid chemistry and recombinant DNA technology
- Includes contemporary purposes of the systems and computations in scientific, educational, commercial, and easy examine laboratories stated through the text
- Features new assurance of electronic PCR and protein quantification together with chromatography and radiolabelling of proteins
- Includes extra pattern difficulties in each bankruptcy for readers to perform concepts

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**Extra resources for Calculations for Molecular Biology and Biotechnology**

**Example text**

From those intersection points on the curve, draw vertical lines to the x axis. 7 h. 7 h Â 60 min/h ¼ 42 min), the generation time is approximately 42 min. 14. 10 PLOTTING CELL DENSITY VERSUS OD550 ON A SEMILOG GRAPH Plotting cell density versus OD550 on a semilog graph allows the experimenter to quickly estimate cell concentration for any OD550 value. 1, this plot gives the curve shown in Fig. 12. 12 Semilog plot of cell concentration versus OD550 for sample data.

5 M NaCl as a percent solution. 44. 5 M NaCl solution. ” This relationship is expressed mathematically as follows: 58:44 g xg ¼ 1M 2:5 M 32 CHAPTER 2 Solutions, Mixtures, and Media Solve for x. 5. 146:1 ¼ x Simplify the equation. 1 g of NaCl are dissolved in a total volume of 1 L. Percent is an expression of concentration in parts per 100. ” 146:1 g xg ¼ 1000 mL 100 mL Solve for x. ð146:1Þð100Þ ¼ x 1000 14610 ¼ x ¼ 14:6 1000 Multiply both sides of the equation by 100 (the denominator on the right side of the equation).

PH ¼ Àlog½Hþ ¼ Àlog 1 Â 10À5 ¼ ÀðÀ 5Þ ¼ 5 Therefore, the pH of the solution is 5. It is acidic. 5 Â 10À4 M. What is the solution’s pH? 18 The product rule for logarithms states that for any positive numbers M, N, and a (where a is not equal to 1), the logarithm of a product is the sum of the logarithms of the factors: loga MN ¼ loga M þ loga N Since we are working in base 10, a is equal to 10. The product rule of logarithms will be used to solve this problem. 6. 5 Â 10À4 on a calculator. 75.

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